Rat Primary Dermal Microvascular Endothelial Cells
- Rat
- Dermal
- Endothelial Cell
- Shipped on dry ice overnight within the US, 2-day international
- CoA per lot on request
- Performance guarantee replaced free if cells fail to attach
For research use only. Not for use in diagnostic or therapeutic procedures.
- SpeciesRat
- TissueDermal
- Cell typeEndothelial Cell
- ModelNormalUnmodified
- FormatFrozen Vial(0.5 x 10⁶ cells)
- MediumM1266Required complete medium
Description
Rat Primary Dermal Microvascular Endothelial Cells
Catalog No. RA-6064
Suggested Medium
M1266 Complete Endothelial Cell Medium w/ Kit – 500 ml
Product Description
Rat Primary Dermal Microvascular Endothelial Cells from Cell Biologics are isolated from the skin tissue of Sprague-Dawley rats and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Rat Primary Dermal Microvascular Endothelial Cells are characterized by use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Storage
Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.
Authorized Uses of Cell Biologics’ Products
Rat Primary Dermal Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
Publication:
J Dent Sci. 2022 Jul; 17(3): 1160–1168.
Am J Physiol Heart Circ Physiol. 2022 Jan 1; 322(1): H71–H86.
Culture protocol in four steps
- 1Thaw37 °C water bath, under 2 minutes. Never re-freeze a thawed vial.
- 2PlateSeed into a flask pre-coated with Cell Biologics coating solution, in M1266 complete medium.
- 3FeedChange medium every 2–3 days until the culture is 80–90 % confluent.
- 4PassageSplit 1:2 with Cell Biologics trypsin; expand for 3–5 passages.
Specification sheet
Source
| Species | Rat |
|---|---|
| Tissue | Dermal |
| Cell type | Endothelial Cell |
| Modification | Unmodified |
Format & QC
| Format | Frozen Vial (0.5 x 10⁶ cells) |
|---|---|
| Also as | T25 Flask, T75 Flask |
| Storage | Liquid nitrogen on arrival |
| Shipping | Dry ice, overnight (US) |
+
M1266
Citations
- Angiogenesis during coronal pulp regeneration using rat dental pulp cells: Neovascularization in rat molars in vivo and proangiogenic dental pulp cell-endothelial cell interactions in vitro
Zar Chi Thein Zaw,a,b Nobuyuki Kawashima,a,∗ Tomoatsu Kaneko,a,† and Takashi Okijia. J Dent Sci. 2022 Jul; 17(3): 116 - Leveraging avidin-biotin interaction to quantify permeability property of microvessels-on-a-chip networks
Feng Gao, Haoyu Sun, Xiang Li, and Pingnian He. Am J Physiol Heart Circ Physiol. 2022 Jan 1; 322(1):
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