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Custom Service Request

NEWS

Cell Biologics provides custom services to our customers upon request.

Adult stem cell analysis (Human and Mouse Cells) 

We provide extensive adult stem cell analysis including hematopoietic stem cells, intestinal stem cells, spermatogonial stem cells and so on.

1. Hematopoietic stem cells: phenotypic analysis, colony-forming assay, spleen colony assay, hematopoieticstem cell transplantation assay, etc.

2. Intestinal stem cells:  phenotypic analysis, differentiation assay including numbers of goblet cells, endocrine cells, Paneth cells, etc.

3. Spermatogonial stem cells: phenotypic analysis, Spermatogonial stem cell transplantation assay. 

Surgical and Functional Assessment Services 

1.     Myocardial infarction and reperfusion

2.      Trans aortic constriction pressure overload model

3.      Mouse Lung orthotopic transplantation

4.      Mouse lung ischemia and reperfusion injury

5.      Mouse kidney ischemia and reperfusion injury

6.      Mouse Skin transplantation

7.      Mouse Parabiosis

Cell Biologics' products can be purchased through Fisher Scientic by searching Cell Biologics' product catalog number or key words...

PROMOTIONS


  • Buy 3 Get 1 Free on Mouse and Rat Hepatocytes.

Promo Code: CBHP0324, before March, 2024

  • Buy 3 Get 1 Free on Mouse and Rat Macrophages

Promo Code: MC0324 before March 2024.

NEW PRODUCT

  • Transfected Stable Cell Lines - Inquiry

  • Gene Knockdown Stable Cell Lines - Inquiry

  • Gene Knockout Stable Cell Lines - Inquiry

  • Gene Overexpression - Inquiry

  • miRNA Overexpression Stable Cell Lines - Inquiry
Featured Cells

C57BL/6-GFP Mouse Primary Kidney Glomerular Endothelial Cells

C57BL/6-GFP Mouse Primary Kidney Glomerular Endothelial Cells
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Price: $0.00
Cat. #: mGFP-6014G

Available Options:
Sequence:
Purity:
Primary Cells:
Qty: Buy Quote

Related Documents:

C57BL/6-GFP Mouse Primary Kidney Glomerular Endothelial Cells

Catalog No. mGFP-6014G

Suggested Medium:  Endothelial Cell Medium /w Kit – 500 ml

Catalog No. M1168

Product Description

C57BL/6-GFP Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are isolated from C57BL/6-Tg (CAG-EGFP) 1Osb/J mouse kidney tissue of pathogen-free laboratory mice. C57BL/6-GFP Mouse Primary Kidney Glomerular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x106 cells per ml and are delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic pre-coated with secondary antibody.

Product Testing

C57BL/6-GFP Mouse Primary Kidney Glomerular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R&D System) or CD31/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. C57BL/6-GFP Mouse Primary Kidney Glomerular Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

C57BL/6-GFP Mouse Primary Kidney Glomerular Endothelial Cells can be used in assays of cell-cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics’ Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.

Never can primary cells be kept at -20 °C.

Authorized Uses of Cell Biologics’ Products

C57BL/6-GFP Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Although C57BL/6-GFP Mouse Primary Kidney Glomerular Endothelial Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

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K57-6014G BKS db Control Mouse Kidney Glomerular Endothelial Cells Buy Quote