C57BL/6 Mouse Primary Alveolar Epithelial Cells
C57BL/6 Mouse Primary Alveolar Epithelial Cells
Catalog No. C57-6053
Suggested Medium
M6621 Complete Epithelial Cell Medium w/ Kit – 500 ml
Product Description
C57BL/6 Mouse Primary Alveolar Epithelial Cells from Cell Biologics are isolated from the lung tissues of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. C57BL/6 Mouse Primary Alveolar Epithelial Cells are characterized by immunofluorescent staining with E-cadherin (Catalog 610182, BD) or ZO-1 (Catalog 617300, Life Technology) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Storage
Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.
Authorized Uses of Cell Biologics’ Products
C57BL/6 Mouse Primary Alveolar Epithelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
Publication:
Cell. 2023 Nov 9; 186(23): 5135–5150.e28.
Cells. 2022 Feb; 11(3): 563.
eLife. 2020; 9: e59961.
Your Review: Note: HTML is not translated!
Rating: Bad Good
Enter the code in the box below:
Have you ever published using C57BL/6 Mouse Primary Alveolar Epithelial Cells? Submit your publication and earn rewards points which can be used for merchandise & discounts. Please include the product used, your name, email, publication title, author(s), PUBMED ID, Journal and issue in your submission.
Title | Author(s) | Journal | Issue |
---|---|---|---|
|
|||
Mechanopathology of biofilm-like Mycobacterium tuberculosis cords | Richa Mishra,1,10 Melanie Hannebelle,1,2,10 Vishal P. Patil,2 Anaëlle Dubois,3 Cristina Garcia-Mouton,4 Gabriela M. Kirsch,1 Maxime Jan,5,6 Kunal Sharma,1 Nicolas Guex,5,6 Jessica Sordet-Dessimoz,7 Jesus Perez-Gil,4 Manu Prakash,2 Graham W. Knot | Cell | 2023 Nov 9; 186(23): |
|
|||
Interferon-γ Preferentially Promotes Necroptosis of Lung Epithelial Cells by Upregulating MLKL | Qin Hao, Sreerama Shetty, Torry A. Tucker, Steven Idell, and Hua Tang* | Cells. | 2022 Feb; 11(3): 563 |
|
|||
A lung-on-chip model of early Mycobacterium tuberculosis infection reveals an essential role for alveolar epithelial cells in controlling bacterial growth | Vivek V Thacker,1 Neeraj Dhar,1 Kunal Sharma,1 Riccardo Barrile,2† Katia Karalis,2 and John D McKinney1 | eLife. | 2020; 9: e59961. |