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Custom Service Request

NEWS

Cell Biologics provides custom services to our customers upon request.

Adult stem cell analysis (Human and Mouse Cells) 

We provide extensive adult stem cell analysis including hematopoietic stem cells, intestinal stem cells, spermatogonial stem cells and so on.

1. Hematopoietic stem cells: phenotypic analysis, colony-forming assay, spleen colony assay, hematopoieticstem cell transplantation assay, etc.

2. Intestinal stem cells:  phenotypic analysis, differentiation assay including numbers of goblet cells, endocrine cells, Paneth cells, etc.

3. Spermatogonial stem cells: phenotypic analysis, Spermatogonial stem cell transplantation assay. 

Surgical and Functional Assessment Services 

1.     Myocardial infarction and reperfusion

2.      Trans aortic constriction pressure overload model

3.      Mouse Lung orthotopic transplantation

4.      Mouse lung ischemia and reperfusion injury

5.      Mouse kidney ischemia and reperfusion injury

6.      Mouse Skin transplantation

7.      Mouse Parabiosis

Cell Biologics' products can be purchased through Fisher Scientic by searching Cell Biologics' product catalog number or key words...

PROMOTIONS


  • Buy 3 Get 1 Free on Mouse and Rat Hepatocytes.

Promo Code: CBHP0324, before March, 2024

  • Buy 3 Get 1 Free on Mouse and Rat Macrophages

Promo Code: MC0324 before March 2024.

NEW PRODUCT

  • Transfected Stable Cell Lines - Inquiry

  • Gene Knockdown Stable Cell Lines - Inquiry

  • Gene Knockout Stable Cell Lines - Inquiry

  • Gene Overexpression - Inquiry

  • miRNA Overexpression Stable Cell Lines - Inquiry
Featured Cells

C57BL/6 Mouse Embryonic Colonic Epithelial Cells

C57BL/6 Mouse Embryonic Colonic Epithelial Cells
Click to enlarge
Price: $0.00
Cat. #: C57-6047E

Available Options:
Sequence:
Purity:
Primary Cells:
Qty: Buy Quote

Related Documents:

C57BL/6 Mouse Embryonic Colonic Epithelial Cells

Catalog No. C57-6047E

Suggested Medium:  Epithelial Cell Medium /w Kit – 500 ml

Catalog No. M6621

Product Description

C57BL/6 Mouse Embryonic Colonic Epithelial Cells from Cell Biologics are isolated from colonic tissue of pathogen-free laboratory mice at embryonic day 14.5-19. Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.

Product Testing

C57BL/6 Mouse Embryonic Colonic Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

C57BL/6 Mouse Embryonic Colonic Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics’ Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C.

Authorized Uses of Cell Biologics’ Products

C57BL/6 Mouse Embryonic Colonic Epithelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

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B129-7047 B129 Mouse Primary Colonic Epithelial Cells Buy Quote