Porcine Primary Hepatocytes -Pleatable

Catalog No. P-6224F 

Custom Cell Isolation for more than 3 vials. 

Suggested Medium: Hepatocyte Medium /w Kit (125 ml)

Catalog No. M1365

Product Description

Porcine Primary Hepatocytes are derived from the the American Landrace Swine liver. Cells are freshly isolated and shipped in 10-50ml cell culture medium overnight at 4°C. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Porcine Primary Hepatocytes are tested for expression of markers using antibodies, ZO-1 by Immunofluorescence Staining. Cells can be plated on culture plates for desired experiments under the cell culture conditions specified by Cell Biologics. Primary hepatocytes can not be cultured indefinitely and can not be passaged. for experiments under the cell culture conditions specified by Cell Biologics. The cells are derived from single donor.

Laboratory Applications

Porcine Primary Hepatocytes can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, metabolism, drug-drug interaction, drug transporter activity toxicity of drug candidates or cell derivatives for desired research applications.

Cell Biologics' Cell Genotype Promise

Cell Biologics has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). Cell Biologics' cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.

Authorized Uses of Cell Biologics Products

Porcine Primary Hepatocytes from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics' Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

Primary Hepatocyte Culture Protocol

All cell culture procedures must be conducted in a bio-safety cabinet.

Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.

Use aseptic technique to prevent microbial contamination.

Medium

Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M1365) to seed cells and when changing media.

Cell Recovery from Cryovial

1. Thaw the cells quickly (<2 min) in a 37°C water bath until just prior to complete thawing.
2. Wipe the outside of the vial with 70% ethanol.
3. Gently resuspend the cells and transfer cells (3 million cells) to a 7 ml sterile tube.
4. Add 7 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M1365, Cell Biologics) gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cells.
5. Carefully pour cell suspension into 100 mm dish (0.6-1.0 million cells per well of 6-well plate).
6. Once the cells have attached to the culture dishes about 70-80% (after 2-8h), gently aspirate supernatant (remove cell debris and non-adherent cells) and replace the pre-warmed Maintenance Medium (Catalog number M1365, Cell Biologics).
7. Cells should be checked daily under a microscope to verify appropriate cell morphology.
8. Proceed with experiment assays within 1-3 days (when cells have reached 90-100% confluence) after plating cells.

Note: The numbers of seeding cells in each well may need to be modified according to the user's experience. Recommended seeding density: 200,000 viable cells/cm².

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