{"title":"Immortalized GFP-Expressing Canine Cells","description":"\u003cp\u003eImmortalized cells from Cell Biologics are derived from primary cells expressing GFP. The primary cells are immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003eThe green fluorescent protein (GFP) gives rise to very bright green fluorescence when exposed to blue light. Cell Biologics generates various GFP-Expressing stable cells, including endothelial cells, epithelial cells and so forth. GFP-Expressing cells are developed through transducing GFP-lentiviral particles.\u003c\/p\u003e\u003cp\u003eCustom order for 1-3 weeks\u003c\/p\u003e","products":[{"product_id":"immortalized-gfp-expressing-canine-primary-colonic-smooth-muscle-cells","title":"Immortalized GFP-Expressing Canine Primary Colonic Smooth Muscle Cells","description":"\u003cp\u003eFor Research Use Only\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eImmortalized GFP-Expressing Canine Primary Colonic Smooth Muscle Cells \u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6001IM.GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2268 Complete Smooth Muscle Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are isolated from the colonic tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized GFP-Expressing Canine Primary Colonic Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of α-smooth muscle actin and negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized cells from Cell Biologics are derived from primary cells expressing GFP. These primary cells are immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Colonic Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580373276,"sku":"D-6001IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-781fc740f5.jpg?v=1791767016"},{"product_id":"immortalized-gfp-expressing-canine-primary-carotid-artery-endothelial-cells","title":"Immortalized GFP-Expressing Canine Primary Carotid Artery Endothelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Carotid Artery Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527580668188,"sku":"D-6008IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-vein-endothelial-cells","title":"Immortalized GFP-Expressing Canine Primary Vein Endothelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Vein Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527581356316,"sku":"D-6009IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-lung-microvascular-endothelial-cells","title":"Immortalized GFP-Expressing Canine Primary Lung Microvascular Endothelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Lung Microvascular Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527581782300,"sku":"D-6011IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-lung-fibroblasts","title":"Immortalized GFP-Expressing Canine Primary Lung Fibroblasts","description":"Immortalized GFP-Expressing Canine Primary Lung Fibroblasts\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527582142748,"sku":"D-6013IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"},{"product_id":"immortalized-gfp-expressing-canine-primary-kidney-glomerular-endothelial-cells","title":"Immortalized GFP-Expressing Canine Primary Kidney Glomerular Endothelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Kidney Glomerular Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527582765340,"sku":"D-6014G.IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-kidney-endothelial-cells","title":"Immortalized GFP-Expressing Canine Primary Kidney Endothelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Kidney Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527582961948,"sku":"D-6014IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-proximal-tubular-epithelial-cells","title":"Immortalized GFP-Expressing Canine Primary Proximal Tubular Epithelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Proximal Tubular Epithelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527583289628,"sku":"D-6015IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-d9b9a1015e.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-kidney-fibroblasts","title":"Immortalized GFP-Expressing Canine Primary Kidney Fibroblasts","description":"\u003cp\u003eFor Research Use Only\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eImmortalized GFP-Expressing Canine Primary Kidney Fibroblasts\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6016IM.GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Kidney Fibroblasts from Cell Biologics are isolated from the kidney tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. These cells are characterized by their spindle morphology and are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing cells from Cell Biologics are derived from primary cells expressing GFP. The primary cells are Immortalized GFP-Expressing by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Kidney Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527583748380,"sku":"D-6016IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"},{"product_id":"immortalized-gfp-expressing-canine-primary-liver-sinusoidal-endothelial-cells","title":"Immortalized GFP-Expressing Canine Primary Liver Sinusoidal Endothelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Liver Sinusoidal Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527584043292,"sku":"D-6017IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-liver-fibroblasts","title":"Immortalized GFP-Expressing Canine Primary Liver Fibroblasts","description":"\u003cp\u003e\u003cstrong\u003eImmortalized GFP-Expressing Canine Primary Kidney Fibroblasts\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6019IM.GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Kidney Fibroblasts from Cell Biologics are isolated from the kidney tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. These cells are characterized by their spindle morphology and are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing cells from Cell Biologics are derived from primary cells expressing GFP. The primary cells are Immortalized GFP-Expressing by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Kidney Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527584338204,"sku":"D-6019IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-16c0aba89f.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-mammary-microvascular-endothelial-cells","title":"Immortalized GFP-Expressing Canine Primary Mammary Microvascular Endothelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Mammary Microvascular Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527584993564,"sku":"D-6020IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-brain-microvascular-endothelial-cells","title":"Immortalized GFP-Expressing Canine Primary Brain Microvascular Endothelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Brain Microvascular Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527585681692,"sku":"D-6023IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-cardiac-microvascular-endothelial-cells","title":"Immortalized GFP-Expressing Canine Primary Cardiac Microvascular Endothelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Cardiac Microvascular Endothelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527586238748,"sku":"D-6024IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-e00077625d.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-small-intestinal-fibroblasts","title":"Immortalized GFP-Expressing Canine Primary Small Intestinal Fibroblasts","description":"\u003cp\u003e\u003cstrong\u003eImmortalized GFP-Expressing Canine Primary Small Intestinal Fibroblasts\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6025IM.GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Small Intestinal Fibroblasts from Cell Biologics are isolated from the small intestinal tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. These cells are characterized by their spindle morphology and are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing cells from Cell Biologics are derived from primary cells expressing GFP. The primary cells are Immortalized GFP-Expressing by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Small Intestinal Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527586664732,"sku":"D-6025IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"},{"product_id":"immortalized-gfp-expressing-canine-primary-prostate-microvascular-endothelial-cells","title":"Immortalized GFP-Expressing Canine Primary Prostate Microvascular Endothelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Prostate Microvascular Endothelial Cells - 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Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527589450012,"sku":"D-6037IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-638de4396d.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-prostate-epithelial-cells","title":"Immortalized GFP-Expressing Canine Primary Prostate Epithelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Prostate Epithelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527589744924,"sku":"D-6038IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-638de4396d.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-stomach-epithelial-cells","title":"Immortalized GFP-Expressing Canine Primary Stomach Epithelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Stomach Epithelial Cells - 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Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized GFP-Expressing Canine Primary Prostate Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of α-smooth muscle actin and negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized cells from Cell Biologics are derived from primary cells expressing GFP. These primary cells are immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Prostate Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527590727964,"sku":"D-6041IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-781fc740f5.jpg?v=1791767016"},{"product_id":"immortalized-gfp-expressing-canine-primary-liver-epithelial-cells","title":"Immortalized GFP-Expressing Canine Primary Liver Epithelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Liver Epithelial Cells - 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Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527592333596,"sku":"D-6046IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-d9b9a1015e.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-colonic-epithelial-cells","title":"Immortalized GFP-Expressing Canine Primary Colonic Epithelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Colonic Epithelial Cells - Canine primary cells isolated and quality-controlled by Cell Biologics for research use.\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527592792348,"sku":"D-6047IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-d9b9a1015e.jpg?v=1791767054"},{"product_id":"immortalized-gfp-expressing-canine-primary-corneal-epithelial-cells","title":"Immortalized GFP-Expressing Canine Primary Corneal Epithelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Corneal Epithelial Cells - 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Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. These cells are characterized by their spindle morphology and are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing cells from Cell Biologics are derived from primary cells expressing GFP. The primary cells are Immortalized GFP-Expressing by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Cardiac Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527594004764,"sku":"D-6049IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"},{"product_id":"immortalized-gfp-expressing-canine-primary-small-intestinal-epithelial-cells","title":"Immortalized GFP-Expressing Canine Primary Small Intestinal Epithelial Cells","description":"\u003cp\u003eImmortalized GFP-Expressing Canine Primary Small Intestinal Epithelial Cells - 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Adult\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCatalog No. D-6067IM.GFP\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSuggested Medium\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eM2267 Complete Fibroblast Medium w\/ Kit (500 ml)\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Dermal Fibroblasts from Cell Biologics are isolated from the skin tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. These cells are characterized by their spindle morphology and are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing cells from Cell Biologics are derived from primary cells expressing GFP. The primary cells are Immortalized GFP-Expressing by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthorized Uses of Cell Biologics’ Products\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eImmortalized GFP-Expressing Canine Primary Dermal Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDisclaimer\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eWarranty and Liability\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eCell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1708462493.pdf\" target=\"_blank\" rel=\"noopener\"\u003eCell Culture Protocol\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e\u003ca href=\"https:\/\/cellbiologics.com\/document\/1698246847.pdf\" target=\"_blank\" rel=\"noopener\"\u003eMSDS\u003c\/a\u003e\u003c\/p\u003e","brand":"Cell Biologics","offers":[{"title":"Frozen Vial (0.5 x 10^6 cells)","offer_id":54527602753820,"sku":"D-6067IM.GFP","price":3200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0937\/8198\/3516\/files\/cb-product-fdeff8eb79.jpg?v=1791766977"}],"url":"https:\/\/cellbiologics.com\/collections\/immortalized-gfp-expressing-canine-cells.oembed","provider":"Cell Biologics","version":"1.0","type":"link"}