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InformationB129 Mouse Primary Aortic Fibroblasts
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B129 Mouse Primary Aortic Fibroblasts
Catalog No. B129-7075
Suggested Medium
M2267 Complete Fibroblast Medium w/ Kit – 500 ml
Product Description
B129 Mouse Primary Aortic Fibroblasts from Cell Biologics are isolated from the aorta of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. B129 Mouse Primary Aortic Fibroblasts are characterized by their spindle morphology and immunofluorescence staining with anti-FSP1/S100A4 (Millipore USA) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Storage
Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.
Authorized Uses of Cell Biologics’ Products
B129 Mouse Primary Aortic Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
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 Fetal Bovine Serum 6912 $0.00 |
 Trypsin/EDTA 0.25% Solution 6914 $0.00 |
 Cell Culture Freezing Medium - ready to use 6916 $0.00 |
Penicillin/Streptomycin Solution (1:100) 6917 $0.00 |
| Antibiotic/Antimycotic Solution (1:100) 6920 $0.00 |
0.1% Gelatin-Based Coating Solution- ready to use 6950 $0.00 |
 Collagen Type I, Rat Tail Solution - ready to use 6953 $0.00 |
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 Complete Fibroblast Medium /w Kit – 500 ML M2267 $0.00 |
| Fibroblast Medium (No Phenol Red) /w Kit – 500 ML M2267PF $0.00 |
Serum-Free Fibroblast Medium /w Kit – 500 ML M2267SF $0.00 |
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