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InformationHuman Primary Kidney Fibroblasts
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Human Primary Kidney Fibroblasts
Catalog No. H-6016
Suggested Medium
M2267 Complete Fibroblast Medium w/ Kit – 500 ml
Product Description
Human Primary Kidney Fibroblasts from Cell Biologics are isolated from human kidney tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Human Primary Kidney Fibroblasts are characterized by their spindle morphology and immunofluorescent staining with anti-fibroblast-specific protein 1 or anti-Vimentin antibody. These cells are negative for mycoplasma, bacteria, yeast, fungi, HIV-1, hepatitis B and hepatitis C and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Storage
Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.
Authorized Uses of Cell Biologics’ Products
Human Primary Kidney Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells received from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
Publication:
J Clin Invest. 2021 Mar 1; 131(5): e143645.
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 Fetal Bovine Serum 6912 $0.00 |
 Trypsin/EDTA 0.25% Solution 6914 $0.00 |
 Trypsin/EDTA 0.05% Solution 6915 $0.00 |
 Cell Culture Freezing Medium - ready to use 6916 $0.00 |
| Penicillin/Streptomycin Solution (1:100) 6917 $0.00 |
Antibiotic/Antimycotic Solution (1:100) 6920 $0.00 |
0.1% Gelatin-Based Coating Solution- ready to use 6950 $0.00 |
 Collagen Type I, Rat Tail Solution - ready to use 6953 $0.00 |
 C57BL/6 Mouse Primary Kidney Fibroblasts C57-6016 $0.00 |
 BKS db Control Mouse Kidney Fibroblasts K57-6016 $0.00 |
 Complete Fibroblast Medium /w Kit – 500 ML M2267 $0.00 |
Fibroblast Medium (No Phenol Red) /w Kit – 500 ML M2267PF $0.00 |
| Serum-Free Fibroblast Medium /w Kit – 500 ML M2267SF $0.00 |
 Fibroblast Basal Medium – 500 ML M2267b $0.00 |
Have you ever published using Human Primary Kidney Fibroblasts? Submit your publication and earn rewards points which can be used for merchandise & discounts. Please include the product used, your name, email, publication title, author(s), PUBMED ID, Journal and issue in your submission.
| Title | Author(s) | Journal | Issue |
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| IL-11 is a crucial determinant of cardiovascular fibrosis | Sebastian Schafer,1,2,* Sivakumar Viswanathan,2,* Anissa A. Widjaja,2,* Wei-Wen Lim,1 Aida Moreno-Moral,2 Daniel M. DeLaughter,3 Benjamin Ng,1 Giannino Patone,4 Kingsley Chow,1 Ester Khin,2 Jessie Tan,1 Sonia P. Chothani,2 Lei Ye,1 Owen J. L. Rackham | Nature | 2017 Dec 7; 552(7683 |
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| Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGF-β signaling in kidney fibroblasts | Yen-Ting Chen 1, Pei-Yu Jhao 1, Chen-Ting Hung 1, Yueh-Feng Wu 2 3, Sung-Jan Lin 2 3 4, Wen-Chih Chiang 5, Shuei-Liong Lin 2 5 6 7, Kai-Chien Yang 1 2 8 9 | J Clin Invest. | 2021 Mar 1; 131(5): |
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| Sphingosine 1-phosphate signaling in perivascular cells enhances inflammation and fibrosis in the kidney | Shinji Tanaka,1,2 Shuqiu Zheng,1 Yugesh Kharel,3 Russell G. Fritzemeier,4 Tao Huang,3 Daniel Foster,4 Nabin Poudel,1 Eibhlin Goggins,1 Yusuke Yamaoka,1 Kinga P. Rudnicka,1 Jonathan E. Lipsey,1 Hope V. Radel,1 Sophia M. Ryuh,1 Tsuyoshi Inoue,1 Junlan | Sci Transl Med | 2023 Jan 24. |
