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Complete Embryonic Fibroblast Medium

Complete Embryonic Fibroblast Medium
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Cat. #: M6821

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Complete Embryonic Fibroblast Medium

Catalog Number: M6821


Embryonic Fibroblast Medium is a complete medium designed for the culture of human and animal embryonic fibroblasts. It was tested and optimized with human, mouse, and rat embryonic fibroblast growth and proliferation in vitro. It is formulated for use with 5% CO2 and 95% air in a humidified incubator. The medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with fibroblast cell growth supplement, antibiotics, and fetal bovine serum.

Cell Culture Medium Supplements include:

- 50.0 ML FBS

- 5.0 ML NEAA (MEM Non-Essential Amino Acids Solution)

- 5.0 ML L-Glutamine

-5.0 ML Penicillin-Streptomycin

*Before use, add Supplements into 435ML DMEM basal medium. For human pluripotent stem cell culture, the Pen/Step could be eliminated.

Storage Condition

Store 500 ml basal medium at 2-6°C. Store cell growth factors, supplements, fetal bovine serum (FBS) and antibiotics at -20°C. The complete cell culture medium with Supplements can be kept in 4°C for two months. Protect from light.

To assure sterility, the prepared medium should be sterilized by filtration through a 0.2 µm filter.


Ice pack and dry ice.

Authorized Uses of Cell Biologics’ Products

Complete Cell Culture Media from Cell Biologics are distributed for in vitro research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

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