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InformationPorcine Primary Skeletla Muscle Microvascular Endothelial Cells
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For Research Use Only
Porcine Primary Skeletal Muscle Microvascular Endothelial Cells
Catalog No. P-6220
Suggested Medium
M1168 Complete Endothelial Cell Medium w/ Kit – 500 ml
Product Description
Porcine Primary Skeletal Muscle Microvascular Endothelial Cells from Cell Biologics are isolated from porcine skeletal muscle tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x106 cells per ml and is delivered frozen. Porcine Primary Skeletal Muscle Microvascular Endothelial Cells are characterized by use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Storage
Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.
Authorized Uses of Cell Biologics’ Products
Porcine Primary Skeletal Muscle Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
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 Endothelial Cell Growth Supplement- ECGS (500X) 1166 $0.00 |
 Fetal Bovine Serum 6912 $0.00 |
Trypsin/EDTA 0.25% Solution 6914 $0.00 |
 Trypsin/EDTA 0.05% Solution 6915 $0.00 |
 Cell Culture Freezing Medium - ready to use 6916 $0.00 |
Penicillin/Streptomycin Solution (1:100) 6917 $0.00 |
Antibiotic/Antimycotic Solution (1:100) 6920 $0.00 |
0.1% Gelatin-Based Coating Solution- ready to use 6950 $0.00 |
 Collagen Type I, Rat Tail Solution - ready to use 6953 $0.00 |
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 Complete Endothelial Cell Medium /w Kit – 500 ML M1266 $0.00 |
Endothelial Cell Medium (No Phenol Red) /w Kit – 500 ML M1266PF $0.00 |
| Serum-Free Endothelial Cell Medium /w Kit – 500 ML M1266SF $0.00 |
 Endothelial Cell Basel Medium - 500 ml M1266b $0.00 |
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