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NEWS


Cell Biologics' products can be purchased through Fisher Scientic by searching Cell Biologics' product catalog number or key words...

Meet Us

Harvard Medical School Feb. 1, 2018 - MA

Experimental Biology April 22-24, 2018 - San Diego

PROMOTIONS


  • Get Free Gelatin-Based Coating Solution per primary cell order, which can be used for most primary cell culture from Cell Biologics through 2018.

NEW PRODUCTS


SHR Rat Endothelial Cells

Cell Biologics provides custom services to our customers upon request. If you have any special needs in cell isolation, please contact us: service@cellbiologics.com.

Thank you!

Cell Biologics

 

  SHR Rat Primary Endothelial Cells       
  (Spontaneous Hypertension)  Frozen Vial     
Catalog No. Product Name 1x106 Cells T25 Flask T75 Flask
SHR-6008 SHR Rat Carotid Artery Endothelial Cells $560 $650 $790
SHR-6009 SHR Rat Vein Endothelial Cells $560 $650 $790
SHR-6011 SHR Rat Lung Microvascular Endothelial Cells $560 $650 $790
SHR-6014G SHR Rat Kidney Glomerular Endothelial Cells $560 $650 $790
SHR-6017 SHR Rat Liver Sinusoidal Endothelial Cells $560 $650 $790
SHR-6020 SHR Rat Mammary Microvascular Endothelial Cells $560 $650 $790
SHR-6023 SHR Rat Brain Microvascular Endothelial Cells $560 $650 $790
SHR-6024 SHR Rat Cardiac Microvascular Endothelial Cells $560 $650 $790
SHR-6027 SHR Rat Prostate Microvascular Endothelial Cells $560 $650 $790
SHR-6052 SHR Rat Aortic Endothelial Cells $560 $650 $790
SHR-6056 SHR Rat Placental Microvascular Endothelial Cells $560 $650 $790
SHR-6057 SHR Rat Spleen Endothelial Cells $560 $650 $790
SHR-6058 SHR Rat Thyroid Microvascular Endothelial Cells $560 $650 $790
SHR-6059 SHR Rat Pulmonary Artery Endothelial Cells $560 $650 $790
SHR-6060 SHR Rat Pulmonary Vein Endothelial Cells $560 $650 $790
SHR-6064 SHR Rat Dermal Microvascular Endothelial Cells $560 $650 $790
SHR-6064L SHR Rat Dermal Lymphatic Endothelial Cells $560 $650 $790
SHR-6065 SHR Rat Retinal Microvascular Endothelial Cells $560 $650 $790
SHR-6090 SHR Rat Ovarian Microvascular Endothelial Cells $560 $650 $790
SHR-6092 SHR Rat Lymphatic Endothelial Cells $560 $650 $790
SHR-6093 SHR Rat Coronary Artery Endothelial Cells $560 $650 $790
SHR-6206 SHR Rat Pancreatic Microvascular Endothelial Cells $560 $650 $790
SHR-6208 SHR Rat Thymus Endothelial Cells $650 $650 $790
SHR-6214 SHR Rat Bladder Microvascular Endothelial Cells $560 $650 $790
SHR-6219 SHR Rat Uterine Microvascular Endothelial Cells $650 $650 $790
SHR-6221 SHR Rat Bone Marrow-Derived Endothelial Cells $560 $650 $790
SHR-6054 SHR Rat Small Intestinal Microvascular Endothelial Cells $560 $650 $790
SHR-6055 SHR Rat Intestinal Mesenteric Vascular Endothelial Cells $560 $650 $790
SHR-6203 SHR Rat Colonic Microvascular Endothelial Cells $560 $650 $790
SHR-6220 SHR Rat Skeletal Muscle Microvascular Endothelial Cells $560 $650 $790
         
M1266  Complete Rat Endothelial Cell Medium 500ml /w Kit $118    
         
  Wistar Kyoto Rat Primary Endothelial Cells       
  Control cells for SHR cells  Frozen Vial     
Catalog No. Product Name 1x106 Cells T25 Flask T75 Flask
WKY-6008 WKY Rat Carotid Artery Endothelial Cells $560 $650 $790
WKY-6009 WKY Rat Vein Endothelial Cells $560 $650 $790
WKY-6011 WKY Rat Lung Microvascular Endothelial Cells $560 $650 $790
WKY-6014G WKY Rat Kidney Glomerular Endothelial Cells $560 $650 $790
WKY-6017 WKY Rat Liver Sinusoidal Endothelial Cells $560 $650 $790
WKY-6020 WKY Rat Mammary Microvascular Endothelial Cells $560 $650 $790
WKY-6023 WKY Rat Brain Microvascular Endothelial Cells $560 $650 $790
WKY-6024 WKY Rat Cardiac Microvascular Endothelial Cells $560 $650 $790
WKY-6027 WKY Rat Prostate Microvascular Endothelial Cells $560 $650 $790
WKY-6052 WKY Rat Aortic Endothelial Cells $560 $650 $790
WKY-6056 WKY Rat Placental Microvascular Endothelial Cells $560 $650 $790
WKY-6057 WKY Rat Spleen Endothelial Cells $560 $650 $790
WKY-6058 WKY Rat Thyroid Microvascular Endothelial Cells $560 $650 $790
WKY-6059 WKY Rat Pulmonary Artery Endothelial Cells $560 $650 $790
WKY-6060 WKY Rat Pulmonary Vein Endothelial Cells $560 $650 $790
WKY-6064 WKY Rat Dermal Microvascular Endothelial Cells $560 $650 $790
WKY-6064L WKY Rat Dermal Lymphatic Endothelial Cells $560 $650 $790
WKY-6065 WKY Rat Retinal Microvascular Endothelial Cells $560 $650 $790
WKY-6090 WKY Rat Ovarian Microvascular Endothelial Cells $560 $650 $790
WKY-6092 WKY Rat Lymphatic Endothelial Cells $560 $650 $790
WKY-6093 WKY Rat Coronary Artery Endothelial Cells $560 $650 $790
WKY-6206 WKY Rat Pancreatic Microvascular Endothelial Cells $560 $650 $790
WKY-6208 WKY Rat Thymus Endothelial Cells $650 $650 $790
WKY-6214 WKY Rat Bladder Microvascular Endothelial Cells $560 $650 $790
WKY-6219 WKY Rat Uterine Microvascular Endothelial Cells $650 $650 $790
WKY-6221 WKY Rat Bone Marrow-Derived Endothelial Cells $560 $650 $790
WKY-6054 WKY Rat Small Intestinal Microvascular Endothelial Cells $560 $650 $790
WKY-6055 WKY Rat Intestinal Mesenteric Vascular Endothelial Cells $560 $650 $790
WKY-6203 WKY Rat Colonic Microvascular Endothelial Cells $560 $650 $790
WKY-6220 WKY Rat Skeletal Muscle Microvascular Endothelial Cells $560 $650 $790
         
M1266  Complete Rat Endothelial Cell Medium 500ml /w Kit $118    

 

Product Description

SHR Rat Endothelial Cells from Cell Biologics are isolated from tissues of male, 9 week old laboratory SHR rats. Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 (CD31) antibody, following the application of magnetic beads pre-coated with secondary antibody.

Product Testing

SHR Rat Endothelial Cells are tested for expression of markers using antibody, PECAM-1 Antibody (M-20, sc-1506, Santa Cruz) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300, Life Technologies) by immunofluorescence staining. Cells are also tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

SHR Rat Endothelial Cells can be used in assays of cell-cell adhesion, migration, vascular tube formation, transendothelial resistance (TER). Standard biochemical procedures performed with cell cultures include RT-PCR, western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C.

Authorized Uses of Cell Biologics’ Products

SHR Rat Endothelial Cells from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution, for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.