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NEWS


Cell Biologics' products can be purchased through Fisher Scientic by searching Cell Biologics' product catalog number or key words...


In December 2017

 

PROMOTIONS


  • Get Free Gelatin-Based Coating Solution per primary cell order, which can be used for most primary cell culture from Cell Biologics through 2018.

NEW PRODUCTS


Culture Macrophages

General protocol for the culture of Primary Macrophages

All cell culture procedures must be conducted in a bio-safety cabinet. Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter. Use aseptic technique to prevent microbial contamination.

Cryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival. Medium: Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML) to recover cryo-preserved cells or when changing media or splitting cells.

Cell recovery from cryovial:

  • Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for <1 min until there is just a small bit of ice left in the vial.
  • Promptly remove the vial and wipe it down with 70% ethanol.
  • Transfer cells from the vial to a sterile centrifuge tube. Add 10 ml of pre-warmed Cell Biologics Cell Culture Medium. Flush the vial with an additional 0.5-1.0 ml of medium to ensure complete transfer of cells to the centrifuge tube.
  • Centrifuge cells at 100 g for 5 minutes.
  • Aspirate the supernatant and resuspend the cell pellet in 5-7 ml of Cell Biologics’  Cell Culture Growth Medium.
  • Add resuspended cells into a plate (tissue culture treated).

Recommended Cell Seeding:

  • 0.7-0.8 million cells are seeded per well of a 12-well plate or 1-1.5 million macrophages are seeded per well of a 6-well plate.
  • Place a plate in a humidified, 5%-CO2 incubator at 37°C until experiments.
  • Change fresh cell culture medium every 24-48 hours.
  • Cells should be checked daily under a microscopy to verify appropriate cell morphology.

Thank you!

Cell Biologics