Newsletter     |     Register     |     Log In     |     Cart(0)     
Shopping Cart
0 items
 

Custom Service Request

NEWS

Cell Biologics provides custom services to our customers upon request.

Adult stem cell analysis (Human and Mouse Cells) 

We provide extensive adult stem cell analysis including hematopoietic stem cells, intestinal stem cells, spermatogonial stem cells and so on.

1. Hematopoietic stem cells: phenotypic analysis, colony-forming assay, spleen colony assay, hematopoieticstem cell transplantation assay, etc.

2. Intestinal stem cells:  phenotypic analysis, differentiation assay including numbers of goblet cells, endocrine cells, Paneth cells, etc.

3. Spermatogonial stem cells: phenotypic analysis, Spermatogonial stem cell transplantation assay. 

Surgical and Functional Assessment Services 

1.     Myocardial infarction and reperfusion

2.      Trans aortic constriction pressure overload model

3.      Mouse Lung orthotopic transplantation

4.      Mouse lung ischemia and reperfusion injury

5.      Mouse kidney ischemia and reperfusion injury

6.      Mouse Skin transplantation

7.      Mouse Parabiosis

Cell Biologics' products can be purchased through Fisher Scientic by searching Cell Biologics' product catalog number or key words...

PROMOTIONS


  • Buy 3 Get 1 Free on Mouse and Rat Hepatocytes.

Promo Code: CBHP0324, before March, 2024

  • Buy 3 Get 1 Free on Mouse and Rat Macrophages

Promo Code: MC0324 before March 2024.

NEW PRODUCT

  • Transfected Stable Cell Lines - Inquiry

  • Gene Knockdown Stable Cell Lines - Inquiry

  • Gene Knockout Stable Cell Lines - Inquiry

  • Gene Overexpression - Inquiry

  • miRNA Overexpression Stable Cell Lines - Inquiry
Featured Cells

Newsletter Subscription

Your Information
* Name:
* Company/Institution:
* E-Mail:

Primary Hepatocyte Culture Protocol

All cell culture procedures must be conducted in a bio-safety cabinet.

Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.

Use aseptic technique to prevent microbial contamination.

Medium

Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M1365) to seed cells and when changing media.

Coating of Cell Culture Plates or Dishes

Coat sterile culture dishes or flasks with Gelatin Coating Solution (Catalog No. 6950, Cell Biologics) for 0.5 min, then aspirate the excess solution.

Handling of Arriving Live Cells

When you receive the live cells in the culture plates

  • Gently aspirate half of supernatant from each well.
  • Place dishes in a humidified, 5%-CO2 incubator at 37°C for 30 min.
  • Aspirate supernatant and add 3 ml of fresh medium of M1365, Cell Biologics for each well of 6-wells.
  • Place dishes in a humidified, 5%-CO2 incubator at 37°C for 8-12 hours (overnight).
  • Next day, wash cells, remove non-adherent cells with PBS, and add the fresh culture medium until experiments.
  • Cells should be checked daily under a microscope to verify appropriate cell morphology.

Cell Recovery from Cryovial

  • Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for <1 min until there is just a small bit of ice left in the vial.
  • Promptly remove the vial and wipe it down with 70% ethanol.
  • Transfer cells (3 million cells) from the vial to a sterile tube and add 7 ml of pre-warmed Cell Biologics’ Cell Culture Medium (Catalog number M1365, Cell Biologics)
  • Carefully pour cell suspension into 2 wells of 6-well-plate (0.7-1.0 million cells per well of 6-well plate)
  • Check on the cells after seeding the cells for 2-4 hours.
  • After cells attached to the culture dishes about 70-80% confluence (and/or 50-80% cells attach to a plate), gently aspirate supernatant (remove the non-adherent cells) and add 3 ml of fresh medium of M1365 for each well.
  • Change culture medium next day and remove non-adherent cells.
  • Cells should be checked daily under a microscope to verify appropriate cell morphology.

Note: The numbers of seeding cells in each well may need to be modified according to user’s experience.

Please send us the cell images (>80-90% confluence) if you have any question or problem with cultured cells.