0.1% Gelatin-Based Coating Solution- ready to use
Gelatin-Based Coating Solution
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular matrices, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue and Type B gelatin is derived from lime-cured tissue. 0.1% Gelatin Solution is used to coat cell culture flasks. Gelatin-Based Coating Solution (from bovine skin) is designed to coat cell culture flasks or dishes for 2 min and then aspirate the excess solution before seeding cells. Aseptically, it dispenses enough gelatin into the dish or flask to completely cover the bottom of the vessel.
Recommended volumes are:
• 3 mL per 25 cm² flask or 60 mm tissue culture dish
• 7 - 8 mL per 100 mm tissue culture dish
The use of coated flasks improves cell attachment for certain types of primary cells.
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