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InformationLactate Dehydrogenase (LDH) Assay
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Lactate Dehydrogenase (LDH) Assay
Cat. #: CB001
Size: 2000 reactions
Kit components:
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Substrate Mix, lyophilizate, store at -20°C protected from light
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Assay Buffer, 0.6mL, store at -20°C protected from light.
Prepare the 2X LDH assay buffer as follows: Dissolve 223 mg INT (2-p-iodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride), 57 mg PMS (N-methylphenazonium methyl sulfate) , 575 mg NAD (nicotinamide adenine dinucleotide), and 3.2 g lactic acid in 480 mL 200 mM Tris buffer solution, pH 8.0.
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10X Lysis Buffer, 9% Triton X-100. Dissolve 9 mL Triton X-100 with 91 mL milli-Q water, 2.5mL, store at 4°C
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Stop Solution, 1 M acetic acid, 12mL, store at 4°C
- LDH Positive Control, 6µL, store at 4°C
Additional Materials Required
• Cultured cell line
• Tissue culture 96-well plate
• Flat-bottom, clear 96-well plate compatible with spectrophotometry
• 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS)
• Multichannel pipette
• Spectrophotometer (ELISA) plate reader capable of reading 490nm and 680nm absorbance.
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