Rat Primary Cardiomyocytes

Catalog No. RN-6368

Suggested Medium: Cardiomyocytes medium/w Kit -125 ml

Catalog No. M6368

Product Description

Rat Primary Cardiomyocytes from Cell Biologics are derived from the heart of pathogen-free laboratory post neonatal mice (day 1-3).  Cells are freshly isolated and shipped at passage 1. Cells are grown in T25 tissue culture flasks, 6-well plates or 12-well plates, which were pre-coated with gelatin-based solution and fibronectin and incubated in Cell Biologics’ Culture Complete Growth Medium for 3-7 days. The beating cardiomyocytes were shipped and ready for customers’ experiments. The method we use to isolate primary cardiomyocytes cells was developed based on a combination of established and our proprietary methods. These cells are purified by removing fibroblasts and endothelial cells. These cells will not proliferate in culture and cannot be passaged.

Product Testing

Rat Primary Cardiomyocytes from Cell Biologics display typical elongated and flattened morphology under light microscopy. Cells are tested for expression of cardiomyocyte marker using antibody cardiac Troponin I (cTnI, Catalog No. ab47003, from Abcam) or Myosin heavy chain (Catalog No. MA5-35613, from Thermofisher. Scientific) by immunofluorescence staining. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased.

Laboratory Applications

Rat Primary Cardiomyocytes can be used for standard biochemical procedures including RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics’ Products

Cell Biologics ships with fresh complete culture medium. On receipt, cells are ready for experimental use. Cells can be maintained up to 7 days under the cell culture conditions specified by Cell Biologics. Subculturing, freezing and thawing of cardiomyocytes are not recommended.  

Authorized Uses of Cell Biologics’ Products

Rat Primary Cardiomyocytes from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Appropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from Cell Biologics with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media (catalog number M6368) and Coating Solution, for appropriate cardiomyocyte culture and cell testing following Cell Biologics’ online protocols within 14 days from the date of product delivery.

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