Porcine Primary Hepatocytes- In Suspension Culture

Catalog No. P-6224S

Custom Cell Isolation for more than 3 vials. 

Suggested Medium: Hepatocyte Medium /w Kit (125 ml)

Catalog No. M1365

Product Description

Porcine Primary Hepatocytes are derived from the porcine liver. Cells at passage 0 are cryo-preserved and each vial contains 6x10⁶ cells per ml. Cells are shipped in 10-50ml cell culture medium overnight at 4°C. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Porcine Primary Hepatocytes are tested for expression of markers using antibodies, ZO-1 by Immunofluorescence Staining. Cells can be cultured in suspension for experiments under the cell culture conditions specified by Cell Biologics. The cells are derived from single donor.

Laboratory Applications

Porcine Primary Hepatocytes can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, metabolism, drug-drug interaction, drug transporter activity toxicity of drug candidates or cell derivatives for desired research applications.

Cell Biologics' Cell Genotype Promise

Cell Biologics has established rigorous genetic quality control and genotyping programs to ensure the normal and genetic background of our cell strains as well as our diseased cell strains with identified diseases (e.g., diabetic cells, hypertentive cells, smoke cells, etc.). Cell Biologics' cell strains are only made available to researchers after meeting our standards. Because it is not possible to fully characterize or capture each cell strain in the cell strain data sheets, we cannot guarantee each cell strain's phenotype will meet all expectations. To ensure that Cell Biologics' cells will meet the needs of individual research projects or when requesting a cell strain that is new to your research, we suggest ordering and performing tests on a small number of cells (1-2 vials) to determine suitability for your particular project.

Authorized Uses of Cell Biologics Products

Porcine Primary Hepatocytes from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics' Cells or Products from the purchaser to other markets, organizations, or individuals is prohibited by Cell Biologics. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

Primary Hepatocyte Culture Protocol

All cell culture procedures must be conducted in a bio-safety cabinet.

Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.

Use aseptic technique to prevent microbial contamination.

Medium

Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M1265) to seed cells and when changing media.

Cell recovery from cryovial:

• Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for <1 min until there is just a small bit of ice left in the vial.
• Promptly remove the vial and wipe it down with 70% ethanol.
• Transfer cells from the vial to a sterile centrifuge tube. Add 5-7 ml of pre-warmed Cell Biologics Cell Culture Medium.
• Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.
• Centrifuge cells at 200 x g for 5 minutes.
• Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.
• Proceed with designed experiments.

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