Canine Primary Hepatocytes - in suspension culture

Catalog No. D-6224S

Suggested Medium

M1265 Complete Hepatocyte Medium /w Kit (500 ml)

Product Description

Canine Primary Hepatocytes from Cell Biologics are isolated from beagle dog liver tissues. Cells at passage 0 are cryo-preserved and each vial contains 5x10⁶ cells per ml. Canine Primary Hepatocytes are characterized by immunofluorescence staining with an antibody of ZO-1. Cells can be cultured in suspention for desired experiments under the cell culture conditions specified by Cell Biologics. Primary hepatocytes can not be cultured indefinitely and can not be passaged.

Storage

Cryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-150°C) immediately until ready for use. Cells should be plated and used directly for desired studies within 1-2 weeks upon receipt. Primary cells can never be kept at -20 °C or -80 °C freezer.

Authorized Uses of Cell Biologics’ Products

Canine Primary Hepatocytes from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

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Primary Hepatocyte Culture Protocol

All cell culture procedures must be conducted in a bio-safety cabinet.

Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.

Use aseptic technique to prevent microbial contamination.

Medium

Review the information provided on the Cell Biologics website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (30-50 ML, Catalog No. M1265) to seed cells and when changing media.

Cell recovery from cryovial:

• Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for <1 min until there is just a small bit of ice left in the vial.
• Promptly remove the vial and wipe it down with 70% ethanol.
• Transfer cells from the vial to a sterile centrifuge tube. Add 5-7 ml of pre-warmed Cell Biologics Cell Culture Medium.
• Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.
• Centrifuge cells at 200 x g for 5 minutes.
• Aspirate the supernatant and resuspend the cell pellet in Cell Biologics’ Cell Culture Growth medium.
• Proceed with designed experiments.

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