BALB/c Mouse Primary Hepatic Stellate Cells

Catalog No. BALB-5242

Suggested Medium

M5569 Complete Hepatic Stellate Cell Medium /w Kit (500 ml)

Product Description

BALB/c Mouse Primary Hepatic Stellate Cells from Cell Biologics are isolated from liver tissues of pathogen-free laboratory BALB/c mice and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Vitamin A droplets in Hepatic Stellate Cells are visualized by blue autofluorescence when excited with UV light. These cells can be used for experiments under the cell culture conditions specified by Cell Biologics. Hepatic Stellate Cells are not recommended for long-term cultures since these cells would differentiate into fibroblat-like cells after plating, and cells will not proliferate in culture and can not be passaged.

Authorized Uses of Cell Biologics’ Products

BALB/c Mouse Primary Hepatic Stellate Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ Cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

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Primary Hepatic Stellate Cell Culture Protocol

All cell culture procedures must be conducted in a biosafety cabinet (BSL-2)

Any and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.

Use aseptic technique to prevent microbial contamination.

Cryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.

Medium

Review the information provided on the Cell Biologics’ website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture medium of M5569 (30-50 ML) to recover cryo-preserved cells and when changing media or splitting cells.

Handling of Arriving Live Cells

Live Cells at passage 0 in a culture plate or flask are shipped overnight. When you receive the live cells in a plate or flask, keep the plate or flask with suggested medium volumn in 37°C CO² incubator for 1 hour before replacing the desired Cell Biologics' cell culture medium.

Note: A recommended media volume for culture dishes, plates and flasks:

6-well plate: Use 2 ml medium per well;

12-well plate: Use 0.5 ml medium per well;

Proceed with experiment assays.

Please send us the cell images (>80% confluence) if you have any question or problem with cultured cells.

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